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This study examined the bypass of γ-OH-PdG by Sulfolobus solfataricus Dpo4, the prototypic Y-family DNA polymerase, using templates that contained the adduct in either the 5′-CXG-3′ or the 5′-TXG-3′ sequence context.
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New VM instances are created from static templates that contain the basic configuration of the VM to achieve elasticity with regards to capacity.
Recent advances in elucidating the molecular mechanisms of carcinogen-induced mutagenesis indicate that replication of DNA templates that contain replication-blocking adducts is accomplished with error-prone DNA polymerases.
Expression from both AUG codons was reduced, indicating that both translation products were derived from mRNA templates that contain both AUG codons.
On 3 April, the synthetic HA coding sequence was cloned into a DNA template that contained the RNA-dependent RNA polymerase genes, SAM vaccine genetic control elements and a T7 polymerase promoter.
Competitors: 'self' indicates the same DNA template without the biotin label, 'GDF' indicates the non-labeled DNA template that contained the Pol III promoter element obtained from the GDF15 promoter, and 'Mut' indicates the Pol III A-box element was mutated in the DNA template.
To further explore the effects of local sequence context on the kinetic signatures of 5mC and 5caC, we used a synthetic SMRTbell template that contained a modified base in a 5'-CG-3' sequence context, surrounded by two random bases on each side.
Semi-quantitative data was obtained from all PCR/ESI-TOF-MS analyses as each well of each assay is seeded with a DNA template that contains the appropriate primer binding sites for the primers in that well.
No-template controls that contained 2 μl of PCR water instead of DNA and positive controls that contained DNA of Brucella melitensis were included in each run to detect any amplicon contamination or amplification failure.
In order to seek confirmation of our hypothesis, we devised a crystallisation experiment with an RNA primer/ DNA template substrate that contained an RNA 9mer, i.e., one base shorter of the primer used in the original crystallization experiment.
A no template control (NTC) that contained all the above reagents was also included to detect the presence of contaminating DNA.
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CEO of Professional Science Editing for Scientists @ prosciediting.com