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This suggests that the metal ions act as bond bridges between functional molecules and silanol groups so that the polymers exhibit very well washing-resistant ability, in comparison to the compact aggregated polymer templates sensitive to washing process [14].
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DOI: http://dx.doi.org/10.7554/eLife.10514.003 We tested whether or not the efficiency of pausing at a nascent RNA length of ∼35 nt on this template was sensitive to the concentration of σ present in vivo by introducing into the cells a second plasmid that did or did not direct the production of excess σ.
However, since Illumina sequencing is highly sensitive to templates presented in DNA samples, some traced transcripts or contaminants can be sequenced in one sample but not in other samples.
A very small template length makes results sensitive to noise while a very large length reduces detection precision.
Unlike PCR, nuclease protection assays do not depend on an exponential amplification process, which is highly sensitive to template secondary structure.
The ESM state in these nano-templates is found to be sensitive to the substrate induced strain.
First, as pointed out by Wu and Zhang [19], the threading performance is usually sensitive to the template library which varies greatly between different methods.
The hollow porous structure was not only sensitive to the soft template but also to the amount of reagents.
Wang et al. found that the mesostructure of MSNFs was sensitive to the AAO template structure and aging conditions [31].
It is easy to see that the MCRS model might be very sensitive to this detail: template directed replication is a dynamical issue requiring closer study.
This was both to control the mutation rate by ensuring that the reaction did not saturate the reagents, which would cause the number of doublings to become sensitive to the initial template concentration, and to avoid the PCR-based recombination events that can occur during with the last few thermal cycles of PCR reactions [ 38, 39].
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