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A template sample manual molding was used.
(a) MB and (b) MO degradation for the three samples: the solution (squares), the solution with the TiO2 flat film (circles), and the solution with the TiO2/Si-template sample (triangles).
As a quality control measure, at least two sample duplicates and a non-template sample per 96-well plate were included.
DNA contamination within the RNA template sample was checked either by use of no RT controls or using primers spanning introns.
Each PCR run included "no template" sample and all tests were performed in triplicate.
Each assay was carried out in duplicate and included a no-template sample as negative control.
As a quality control measure, we included at least 2 sample duplicates and 1 non-template sample per 96-well plate.
The 200 ng gDNA template sample was amplified separately after evaluation of the genotyping performance of the wgaDNA produced from 1 – 100 ng gDNA.
Since the human oocyte is a low RNA template sample which contains approximately 55 to 100 pg of polyA+ mRNA [ 20, 21], it was proposed that an amplification step is required to provide sufficient labeled RNA as a microarray target.
Inclusion of a template sample from the non-domesticated species PS3689 (wild type landrace accession of Pisum sativum from Afghanistan) permitted polymorphism detection by 11 additional primer pairs (an increase to 59.3% of total) (Additional file 15, sheet Fieldpea).
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CEO of Professional Science Editing for Scientists @ prosciediting.com