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At 95 SNPs, comparison of TaqMan genotypes generated from MDA product and gDNA templates revealed a very good concordance rate but a higher failure rate for MDA product compared to gDNA.
RT-PCR assays using extracted RNA from normal and scrapie-infected hamsters as the templates revealed a 187 bp product with Tau-2-specific primers and a 127 bp fragment with Tau-4-specific primers, possibly representing the tau isoform of 372 amino acid residues and isoform of 432 amino acid residues, while the primers for Tau-6 failed to produce positive product (data not shown).
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qRT-PCR reactions using the cDNA as a template revealed a shift in the amplification curve between reactions using either the native luc or synthetic luc* primer pairs.
In the related species V. pannonica, initial investigation of the VicTR-A repeats, using probe amplified in a PCR reaction with genomic DNA as a template, revealed a different hybridization pattern for their eccDNA, consisting of the smear with spots spaced by about 180 bp which could not be derived from multiples of the monomer length (Fig. 3J).
Homology modelling of soft (RR-1) cuticular proteins using bovine plasma retinol binding protein (RBP) as a template revealed an antiparallel β-sheet half-barrel structure as the basic folding motif (Hamodrakas et al., Insect Biochem. Molec. Biol. 32 (2002 15777).
A TEM observation of these templates revealed that all of them possessed a 2-D hexagonal mesoporous structure with the same caliber of ca. 7 nm (Figures 1B, C, D).
A direct comparison of the root- and leaf-extracted DNA templates revealed that the former contained fewer (average of 26-fold) plastome reads, but a greater number of reads from bacterial genomes (average of 28-fold).
Repetition of this cDNA concentration experiment with other templates revealed that exact reproduction qPCR experiments was affected by the time it takes to complete the pipetting of a qPCR plate.
As both DNA templates revealed similar results of large proportions of amplified genes, it is reasonable to assume that the lack of expression of the various multicopy gene families in the ex vivo sample as well as during the first 12 days of culture was not the result of biased amplification.
A scatterplot of QFI-PCR and Qubit DNA template functionality revealed a linear trend for templates with at least 3% QFI by qPCR (R = 0.66; N = 86).
Thin films of this material were synthesized by block copolymer template-directed, evaporation-induced self-assembly, resulting in a periodic orthorhombic structure with Fmmm symmetry that, after template removal, revealed a pore size of around 7.5 nm.
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