Suggestions(2)
Exact(3)
However, when biomass from pure cultures of C. chauvoei or tissue extracts were used as templates, only the primer pair designed to produce the 159 bp amplicon gave consistently positive results.
To minimize anti-V region responses, the Ab may be humanized by grafting onto the human templates only the specificity-determining residues (SDRs), the residues that are essential for the surface complementarity of the Ab and its ligand.
After successful amplification of the 522 bp plasmid fragments from templates only, the presence of the amplicon was confirmed on 1.5 % TBE gel, purified and sent off for confirmatory dideoxy sequencing.
Similar(57)
The Oct4 amplification plots generated by the same amount of genomic DNA in the presence of different Taq polymerase concentrations are reported in Fig. 2. The thin lines were obtained from amplification of Oct4 templates only, while the thick lines show amplification of Oct4 in the duplex assay.
Although all DNA ligases tested could efficiently join multiple oligos hybridized to the DNA template, only T4 DNA ligase and RNA ligase 2 (Rnl2) joined the DNA oligos when hybridized to the RNA template.
The template only tells the kind of event, while your message conveys a personal tone and lets people know if they need to bring anything.
It was not possible to generate a homology model because the available templates only included 279 amino acid residues.
(A simplified version of this assay was employed for the initial measurements of Oct4 templates only.
These templates only function on your main User page.
To speed-up, our method first aligns a target to all the templates using only the local alignment potential and then ranks all the templates using both the local and global alignment potentials described in Section 2. After ranking, only the first-ranked templates are used to build a 3D model by MODELLER for each target.
mtDNA with the 4977 or 4839 bp deletion would serve as a template only for the mtR8416 and mtR13738 primer sets and yield a 346 or 485 bp PCR product, respectively.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com