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However, when biomass from pure cultures of C. chauvoei or tissue extracts were used as templates, only the primer pair designed to produce the 159 bp amplicon gave consistently positive results.
To minimize anti-V region responses, the Ab may be humanized by grafting onto the human templates only the specificity-determining residues (SDRs), the residues that are essential for the surface complementarity of the Ab and its ligand.
(A simplified version of this assay was employed for the initial measurements of Oct4 templates only.
It was not possible to generate a homology model because the available templates only included 279 amino acid residues.
Colors are used to distinguish templates where the forward and reverse fragments are in separate contigs, have an inconsistent (unexpected) orientation or are single-ended sequencing templates only.
After successful amplification of the 522 bp plasmid fragments from templates only, the presence of the amplicon was confirmed on 1.5 % TBE gel, purified and sent off for confirmatory dideoxy sequencing.
Similar(50)
In the proposed method, a key sequence is used for a template only.
For practical purpose, each type of label is confined to one template only which allows automated packing and handling.
In the Profile Template, only Aims and Results are specifically cued and can be seen to be recorded.
The lower height value suggests that the structure is templated only by HOPG in which the carboxylic acid carbon plane faces HOPG [4, 53].
The template only takes a few minutes to complete including noting the student details, circling irregularities and brief notes about examples.
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CEO of Professional Science Editing for Scientists @ prosciediting.com