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To obtain such scan-specific attenuation maps, registration-based methods have been proposed, suggesting to incorporate pre-acquired attenuation templates of the corresponding hardware components into the vendor-provided attenuation maps.
In contrast to all other methods proposed for AC of flexible hardware components [22, 25, 27 29, 33, 35], our method does not rely on the co-registration of pre-acquired attenuation templates of the corresponding hardware but estimates the attenuation directly from the PET emission data.
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A transmission scan using computed tomography (CT) or rotating rod sources is performed to obtain an attenuation template of the corresponding hardware component [21 24, 30 32].
In order to validate the efficacy of allelic specific primers to accurately discriminate between alleles within a cDNA population it was necessary to demonstrate the primer specificity on the DNA template of the corresponding allele.
> -wrap-foot> Another search option allows retrieval of some 100 pig sequences that can be modelled with 67 cluster HMMs whose structural templates are derived from distantly related prokaryotic sequences (the list of these templates and the corresponding pig protein is included as Supplementary Material).
The sequence identity between the helix 7 of the template and the corresponding helix of the target is only 6%, and the global sequence identity is only 27%.
To assure the integrity and to control the load of cDNA, glyceraldehyde-3-phosphate dehydrogenase was amplified with primers GAPDH-F (5′-TGGAAATCCCATCACCATCT) and GAPDH-R (5′-GTCTTCTGGGTGGCAGTGAT). PCR was run in a 23 μl reaction in the ready-to-use Supermix PCR mixture (Invitrogen) containing 3 μl of template and the corresponding primers at 0.22 μmol/l.
Only the elements that have no missing ORFs when compared to the IS templates, called full copies of coding regions of the corresponding IS templates, are kept for further analysis.
The absolute mRNA levels in each sample were calculated according to a standard curve determined using serial dilutions of known amounts of specific templates plotted against the corresponding cycle threshold (CT) values.
A variety of amorphous manganese oxide electrode materials with uniform nonspherical hollow interiors are prepared via sacrificial template-engaged redox etching of the corresponding shape-controlled Cu2O nanocrystals in KMnO4 aqueous solution at room temperature.
The assembly of the half-proteasomes, in turn, is initiated by the assembly of the α subunits into their heptameric ring, forming a template for the association of the corresponding pro-β ring.
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