Sentence examples for templates of second from inspiring English sources

PCR reactions were performed as follows: one cycle at 94°C for 4 min; 25 cycles at 94°C for 30 s, 56°C for 40 s and 72°C for 30 s; followed by one cycle at 72°C for 10 min. The first round PCR products were used as the templates of second round inverse PCR after 100 fold dilution.

Total RNA (1 μg) was used for template of first-strand cDNA synthesis using extent reverse transcription kit (PrimeScript RT reagent Kit with gDNA Eraser, TaKaRa, Japan).

The Guardians Michael Cragg called "Trouble" a return to the "safer" templates of her first two albums, but praised the song's production calling it "a vaguely trip-hop beat buffeted by soaring strings and a troubled lyric in the chorus".

As the GeneChip system utilizes polyadenylation complementary oligonucleotides as a primer for reverse transcription of the starting RNA template, inefficiency of first strand cDNA synthesis and/or in vitro transcription of cRNA can result in under representation of the 5' moiety of the transcript [ 34, 52].

The 140 fold diluted samples were used as a template for second step of PCR reactions.

A 1-μl aliquot of the first-round PCR product was used as the template of the second-round PCR reaction.

Romy Madley Croft, Oliver Sim and Jamie Smith may have stuck close to the template of their first album – Coexist is yet another barely there album in which the dawn seems far away – but it's hard to imagine anyone minding, so compelling is their signature sound.

Using the template of the first PCR reaction, allele-specific primer pairs was used to test for the presence of the allelic variants from FC27 and IC of the families of the msp2 region.

First round PCR was done with primers PDS155 (5' - ATT CAC CGC TGC TGC TTA AA - 3') and AKR1-KPK (5' - TAG GAA AAT GGC TCG GTG TC - 3') for 19 cycles. 2 ul of first round PCR product was diluted into 18 ul of water, and 1.5 ul of this dilution was used as template for 35 cycles of second round PCR.

Double stranded cDNA was synthesised using the Smart ™ cDNA library construction kit (Clontech UK): single-stranded cDNAs were obtained by reverse transcription, tagged by the SMART IV oligonucleotide, which allow template switching and synthesis of second strand by the reverse transcriptase.

RNA samples with distinct 18S and 28S ribosomal RNA bands on denaturing agarose gels were then used as templates for synthesis of first strand cDNAs as described previously (6, 9).