This requirement stems from the fact that reagents included with the kits contain contaminating DNA that compete with the amplification of template of interest [ 11].
After warping the images to the final template, region of interest drawing was completed on the template through the Anatomical Automatic Labeling Toolbox [29].
A Hoffman phantom template (volume of interest indicating grey and white matter volume) was co-registered to the reconstructed images, and grey matter time activity curves (TACs) were extracted.
The quantitation of the competitor templates or samples of interest is largely performed in a spectrophotometric manner; that is, the method takes into account the common measurement biases that occur between measurements and between laboratories.
Given the success of our klp-12 disruption experiment, we constructed a set of vectors to facilitate cloning of new repair templates for genes of interest while providing flexibility and diversity of available epitope and fluorescent protein tags (GFP, mCherry, 3xHA, and GFP 3xHA tags).
Ms. Zalaznick had a revelation: The categories of the "Queer Eye" cast members seemed to offer a template for areas of interest that Bravo could speak to: food, fashion, design, beauty and pop culture.
A template of regions of interest (ROIs) [15] was used to extract brain time-activity curves for the following 13 regions: anterior and posterior cingulate cortices, caudate, putamen, thalamus, pons, cerebellum, hippocampus, parahippocampus, and the prefrontal, parietal, temporal, and occipital cortices.
MIPs and nonimprinted polymers were weighted into polypropylene microtubes and the solution of the template (or analyte of interest) was pipetted on the particles in toluene or in acetonitrile.
Several automated approaches to MRI morphometry have been developed over the past decade, including template-based, region of interest (ROI) labeling procedures (e.g., see [ 39- 42]), cortical thickness approaches [ 43, 44] and voxel based morphometry (VBM [ 45- 47]).
In this assay, we use PCR based mutagenesis to produce T7 templates including the mutations of interest, and then use these DNAs to produce the mutant RNA templates.
