Using these primers, amplicons were derived from a DNA template pool consisting of equal quantities of genomic DNA from 96 individuals of the Lp1 mapping family (effectively recreating the genomic constitution of the original F1).
Self assembly of silver nanoparticles grown on tryptophan template leads to emergence of equal intensity double plasmon resonance (EIDPR).
External spike-in controls are not assumption-free; it is assumed that the experimenter starts with the same quantity of equal quality template.
This includes droplet digital polymerase chain reaction (ddPCR) systems (e.g., Bio-Rad QX200) that disperse template DNA randomly into emulsion droplets of equal volume.
First-strand cDNA were reversely transcribed from mRNA templates of each skin sample in equal amount, using MMLV High Performance Reverse Transcriptase Kit with an oligo dT) primer (EPICENTRE Biotechnologies, Madison, WI).
The number of templates, r, is equal to j+1.
A second nail of equal length was used as a template and guide for proximal locking and determining the site of the anterolateral cortical window.
PCRs for the housekeeping gene GAPDH served as a positive control for the RT reactions and the use of equal amounts of cell line-specific template RNA (Figure 2B).
The transcription/translation reactions of equal amounts the Apobec1, Apobec2 and Apobec3 templates generated [35S]methionine-labeled proteins of the expected sizes, as determined by electrophoreses.
Primers selected in table 1 were of equal efficiency (Eff = 1.9) across the range of template concentrations (1 10 ng cDNA).
