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Eventually, Amazon may enable developers to monetize templates listed in the repository.
Other constructs were performed in two PCR steps using the primers and templates listed in Table S1.
All reporter genes, except for the lux operon, were PCR amplified using the primers and templates listed in Table 5.
The list of E.coli pCoofy vectors was extended by modifying additional pETM vectors or by introducing His10, OneStrep, S or Halo tags from templates listed in Table 1.
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The results of N2 adsorption/desorption isotherms of meso-SAPO-11 by different contents of FW200 and Carbot templates are listed in Tables 2 and 3.
Primers used for amplification of templates are listed in Additional file 10: Table S2.
The oligonucleotides used to amplify the according probe templates are listed in Supplementary Table S3.
Template models are loaded from a directory containing one ASCII file per template model, listed in the ASCII index file, "templ_index.txt".
The oligonucleotides used for the sgRNA template are listed in Supplementary Data 1.
Quantitative PCR was performed using this cDNA as a template, primers listed in Supplementary Material, Table S3, Brilliant II SYBR Green QPCR Master Mix (Agilent Technologies) and a CFX96 Real-Time PCR Detection System (Bio-Rad).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com