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For the proposed method, it started with the initialized Guard template, and the required number of templates is determined adaptively during the analysis process.
The wetting behaviour of the templates is determined by the hydrophilicity of the surface, the wetting properties of the solution and the contained nanoparticles.
Hence, the sequence of the DNA templates is determined from the knowledge of their location, the order of the flow of nucleotides and records of each flash of light from each well [ 10– 10].
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The amount of carbon templates was determined by 5 wt% weight ratios of those to theoretical yield of SAPO-11.
The concentration of templates was determined by qPCR with Library Quantification Kits from KAPA biosystems.
The linear range of PCR templates was determined by performing a 10-fold serial dilution standard curve, which usually proved a 1 10 dilution was sufficient.
Steady-state kinetic parameters for incorporation of the nucleotide opposite the unmodified and FAF- or FABP-modified templates were determined by following the procedures reported previously.
The copy number of rDNA operons of targeted bacteria in crude DNA templates was determined by comparison with serially-diluted plasmid DNA standards run on the same plate.
To determine the efficiency of dCTP insertion opposite the adducted site, steady-state kinetic parameters for incorporation of the nucleotide opposite the unmodified and FABP-modified templates were determined by using the reported literature procedures.
The optimal position of the template is determined by {k}_{mathrm{opt}}=underset{k}{arg max}left(c k)right) (9).
After the best template was determined, fold consistency within the template candidates was considered using TM-score and SCOP database to select additional template structures among the template library.
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