The head template size is dependent on both subject and view; in this figure, the template sizes of the original image are 30 × 26 and 26 × 20 pixels for the 85° and 55° views, respectively.
To examine whether the specificity of template-switching is dependent on temperature [ 14], we varied in the next experiment the temperature of the reverse transcription from 37 to 57°C.
Thus in contrast to dsDNA dyes, no fluorescence is generated from amplification of nonspecific templates because the fluorescent signal is dependent on hydrolysis of the probe following hybridization to a specific target sequence.
Because the optimal potassium concentration is dependent on the template, each construct was evaluated in the same manner.
The use of adaptamer sequences with identical Tm's for the 2nd round of amplification optimized the yields, despite different product lengths and different template concentrations (which were dependent on the yield from the first round reactions).
The number of protein molecules synthesized using a given mRNA molecule as a template is highly dependent on translation-initiation features of the mRNA sequence.
In this initial study, however, we greatly simplify the system by assuming that replicases do not discriminate between inter-species and intra-species replication, so that the replication rate constants, k SP, k SM, k DP and k DM, are dependent solely on templates.
The plots clearly showed that the degree of variability was dependent on the template concentrations.
However, some questions and problems remain in relation to the template-based methods: (i) the prediction accuracy might be dependent on the number and types of atoms in the templates.
The electrocatalysis was dependent on the size of the template, being more pronounced at 50 nm than at 100 nm.
