Sentence examples for templates is added from inspiring English sources

A standardized mixture of internal standard (SMIS) competitive templates is added to the reverse transcribed products prior to PCR.

Application of this method permitted specific genotype identification for all cases with no cross-reactivity, even when both templates were added to the same tube.

Then, 2 µL cDNA templates were added to duplicate reactions performed in 96 well plates.

It was found that the average TM-score [31] of the first identified templates can be increased by 2.5% with the introduction of the torsion angle restraints, where the difference between ANGLOR-predicted angles and the experimental angles in templates was added to the profile-profile alignment scores.

Primers and templates were added subsequently.

DNA was extracted, reaction vials were prepared for amplification, templates were added, and products underwent electrophoresis in separate rooms.

In order to reduce pipetting variability, we performed reaction batches containing primer pairs, and templates were added in the end.

Additionally, when stitching, the templates were added in equal molar ratios and PCR primers were added after the initial five cycles.

Each mastermix for PCR was prepared in a clean room separated from where the DNA templates were added; no research with R. honei TT-118 had been conducted in our laboratory.

Included in each run were 3 negative controls (GIBCO Ultrapure DNA-free distilled water, Invitrogen Corporation, Grand Island, NY, USA), 1 produced in the clean room and 2 in a biosafety cabinet in another laboratory where DNA templates were added.

The genomic DNA templates were added to a master mix containing 2 μl of each primer (10 μM), 5 μl Buffer (New England Biolabs, Ipswich, MA), 2 μl of Taq polymerase (Invitrogen), and water to the volume of 50 μl.