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For real-time multiplex RT-PCR, if one template is much more abundant than the other templates in the same reaction tube, the reaction components will be depleted before the lower-abundance targets have amplified sufficiently to be detected [23].
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It included spatial normalization parameters in statistical parametric mapping (SPM 2 (http://www.fil.ion.ucl.ac.uk/spm/) and a [99mTc]ECD brain template in the same space as the Montreal Neurological Institute (MNI) standard brain template.
We thus constructed a template in the same modality as the images we wanted to normalize and we spatially normalized raw FA images (with linear affine and nonlinear parameters) to this modality-specific template.
The primers were designed to amplify both methylated and unmethylated template in the same reaction.
RNA was prepared using the Promega RiboMax™ T7 system (Promega), where sense and antisense strands were transcribed from DNA template in the same reaction.
Due to its multiplex format, ResPlex III can detect more than one viral template in the same reaction mixture, allowing viral co-infections to be identified in the same specimen.
By significantly increasing the non-overlapping region the primers should be long enough to bridge the "nick" and bind to the newly synthesized DNA and thus use it as template in the same way as normal PCR.
In our work, we employed the position-dependent gap penalty model in the dynamic programming, which operates as follows: No gaps are allowed in the region where the predicted secondary structure and the secondary structure of the template are in the same state of helix or sheet; The end gap penalty is neglected; Affine gap opening (go) and gap extension penalties (ge) are applied to other regions.
For inversions, the data is also consistent with a predominantly intramolecular mechanism in which a 3'-end relocates to a new template contained in the same branched DNA molecule (Fig 3A and 3B).
Templates were generated in the same way as for the arms.
This advantage is due mainly to the fact that the removal of aluminium oxide from the pore bottom tips in the NAAM template takes place in the same electrolyte in which the anodization is carried out.
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