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DNA templates generated by PCR were labeled with 32P using the Megaprime DNA labeling system (Amersham Biosciences, England).
Significance for the prediction is compued as a nominal p-value estimated based on a null distribution for the distance d to the templates generated by randomly resampling nA+nB genes from the N genes 1,000 times (default).
Alternatively, the (+31) 'leadered' mutants of DVxrRNA1 were made using DNA templates generated by PCR amplification of overlapping primers.
DNA templates generated by shearing had an initial size range of 100 500 bp (data not shown).
Every primer set was quality-checked (QC) using reference cDNA templates generated by reverse transcription of the microarray experiment reference RNA.
DG templates generated by restriction enzymes that are methylation sensitive have relatively low coverage in pericentromeric heterochromatic regions of sorghum chromosomes.
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A circular template generated by enzymatic ligation upon the recognition of a point mutation (CGT to CAT) on the oncogene could be amplified isothermally by Phi29 DNA polymerase.
In [15], decision-directed autocorrelation (DDA) receivers are proposed to detect the current symbol by correlating the current information waveform with a waveform template generated by all previously decoded symbols.
This process iteratively matches selected images to a template generated by their own mean.
GPs will print-out the completed risk-assessment template (generated by the CARAT) for subsequent review by the project officer.
For sgRNA synthesis, the T7 promoter sequence was added to sgRNA template/forward primer and the IVT template generated by PCR amplification using primers listed in Table S5.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com