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We hypothesized, however, that the homology between the human template sequences and mAb to be humanized may be maximized by using templates from multiple human germline sequences corresponding to the different segments of the variable domain.
Transcriptome sequencing was performed with RNA templates from multiple tissues of the field pea genotypes Kaspa and Parafield.
Candidate genes from the RNA-seq results were validated by qRT-PCR against templates from multiple genotypes, developmental stages, and/or environments.
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Until now, however, individual emulsion PCR (emPCR) reactions and subsequent sequencing runs have been unable to combine template DNA from multiple individuals, as homologous sequences cannot be subsequently assigned to their original sources.
They speculate that their CPOE's connection to serious errors is a "result of physicians choosing the wrong drug template, selecting from multiple options, or as a consequence of constructing their own drug prescriptions using pull down menus".
Dose errors were still prevalent with CPOE, however, as a result of physicians choosing the wrong drug template, selecting from multiple options, or as a consequence of constructing their own drug prescriptions using pull down menus.
In order to further develop an international core dataset, a consensus-driven revision of the Utstein Trauma Template, with representatives from multiple continents, should be initiated.
In total, roughly 45% of the targets that a model created from multiple templates are better than the best single-template model.
Thus, using a balanced consensus of template domain assignment and the internal and terminal gaps from multiple template alignments, the DCS system helps erase the incident errors from single template alignment by individual threading programs that are often less reliable.
But in mapping out the relationships among the papers, the duo noticed that the writers seemed to be drawing from multiple templates.
The estimation and prediction framework offers a template for using data from multiple cases to estimate both case-specific and industry-wide parameters of project models, and for leveraging those estimates for project planning.
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