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The detection range of the method was assessed on purified DNA templates from different animal origins.
The use of NEMA keys allows templates from different structures to be compared, and allows storage of structures containing a canonical list of templates.
Open image in new window Fig. 1 Agarose gel showing polymerase chain reaction (PCR) results using DNA templates from different governorates with the primer sets LCO1490/HCO2198 for COI.
This observation motivates us to propose a Spectral Feature Alignment (SFA) based method to align workflow DS templates from different domains in a latent space by modeling the correlation between the DI and DS workflow templates in a bipartite graph and using DS features as a bridge for cross-domain Big Data classification.
Thus, it has been suggested that combination of templates from different levels of MNase digestion may alleviate biased sampling of mononucleosome populations [ 58].
In order to confirm the RNA slot blot, internal primers were designed from each fragment (Additional file 4) and RT-PCR was conducted using cDNA templates from different tissues following standard thermal profiles (Additional file 4).
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MASA reactions were performed using cDNA samples as template from different tissues and spermatozoa following standard procedure [ 38, 39].
RT-PCR amplification of template RNA from different samples was performed using ExTaq kit (TaKaRa) using manufacturer's protocol.
The use of these colloidal crystals as templates for different microstructures range from nanoscale to micron-scale is also summarized.
In addition to careful quantitation of input genomic DNA, q-PCR could be used following digestion and ligation of bar-coded adapters to normalize template numbers derived from different genotypes at the template pooling stage [ 35].
Our model allows us to investigate sampling designs in the frequently occurring scenario in which imputation targets and templates are sampled from different populations.
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