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While this dataset [36] was also generated by PCR, the incorrectly-processed molecules were in greater abundance (permitting PCR cycle number to be small), because the process of rearrangement had been experimentally stalled or halted by depleting specific RNA templates for two independent genes via RNAi.
The cDNA dilutions of 1/10 (10 cells), 1/10 (10 cells), 1/10 (10 cells), 1/10 (one cell) were utilized as templates for two rounds of RT PCR amplification of MAGE-1 and MAGE-3 transcripts.
The digested DNA was then ligated with a DNA adaptor (Clontech) and the resulting DNA fragments were used as templates for two rounds of primary and nested PCR amplifications using two adaptor-specific primers together with two myog specific primers (GSPs) in the first exon (GenBank accession number EU934044).
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Design templates for five primary schools, five secondary schools and one all-through school have been created by 11 leading architectural practices, including Marks Barfield, the architects who gave us the London Eye; Alsop & Partners; Sarah Wigglesworth and Wilkinson Eyree.
Nor will they care that to make "Polar Express" Mr. Hanks wore a little cap that transmitted a record of his movements to a computer, creating templates for five different animated characters.
Nor will they care that to make "Polar Express" Mr. Hanks wore a little cap that transmitted a record of his movements to a computer, creating templates for five animated characters.
We performed this study to assess the reproducibility and accuracy of the templates for three commonly used knee implants (PFC, Kinemax, Scorpio).
This paper highlights the application of the templates for three case studies: (i) the design of a refrigeration cycle, (ii) a mixture/blend design problem involving lubricant design and (iii) a tailor-made product design of jet-fuels (blended chemical products).
In Fig. 2A we show the heartbeat templates for one muscle (lateral rectus in m3); each trace corresponds to a different muscle length.
All known experimental BRD structures (23 X-ray and 3 NMR) were downloaded from the June-2009 PDB release and used as comparative modeling templates for eleven yBRDs.
Briefly, cDNA was generated using Superscript III with random hexamers (Invitrogen), which provided templates for five independent PCRs.
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