Sentence examples for templates for sequence from inspiring English sources

In this study we demonstrate that modifications to the Applied Biosystems sequencing protocol allow unpurified AFPs to serve as templates for sequence identification (Fig. 1B).

To generate DNA templates for sequencing, PCR reactions were done using the Elongase Amplification System (Invitrogen, Glasgow, UK).

Amplified fragments were then cloned using the pGEM-T easy Vector System (Promega) and used as templates for sequencing.

The clonally amplified beads, each representing a unique genomic DNA fragment, are used as templates for sequencing.

Both gel-purified PCR products and cloned inserts which were confirmed in the range of about 700 bp were used as templates for sequencing.

The PCR products were used as templates for sequencing reactions with the BigDye Terminator kit v3.1 (Applied Biosystems, Foster City, CA, USA).

Vector primers were used to generate the final DNA templates for sequencing.

The sequencing involved cloning of ten representative RT-PCR products into a recombinant plasmid to obtain templates for sequencing.

dMS-HRM can also rapidly generate clonal templates for sequencing or pyrosequencing, eliminating the need for conventional cloning.

For this purpose, both genomic DNA and genic regions (using cDNA libraries) have been used as templates for sequencing.

The PCR products were purified with a Multiscreen plate (Millipore) and used for templates for sequencing with the primer amoA359rC.