Sentence examples for templates for real from inspiring English sources

Templates for real time PCR were made by Cloned AMV Reverse Transcriptase (Invitrogen).

A series of dilutions were prepared that contained defined numbers of standard molecules, and 5 µl aliquots were used as templates for Real Time PCR.

cDNAs were used as templates for real time PCR.

Aliquots containing equal amounts of total RNA were used as templates for Real Time PCR analysis with the GUS specific primerset.

The input and immunoprecipitated DNA were used as templates for Real Time PCR preformed using the primers corresponding to the promoter of the HSD17B1 gene (Additional file 1, Additional file 2).

The input and immunoprecipitated DNA were used as templates for Real Time PCR performed with MYCT1 core promoter-specific primer amplifying the second c-Myc binding regions (c-Myc B, F 5'-GAGGTCAGGCCTAGTTCATG-3', and R 5'-CTTAGT CTCGCTCTGTCGC-3') in accordance with RQ-PCR except the difference of 40 cycles.

First-stranded complementary DNAs (cDNAs) were synthesized from 0.5 μg of the isolated RNA by oligo(deoxythymidine) (oligo (dT)) using the DyNamoTM cDNA Synthesis Kit (Fermentas) and used as templates for real-time PCR.

The cDNA was 20 × diluted and used as templates for real-time PCR.

The RNA sampled at four times during bud dormancy provided templates for real-time quantitative PCR (Q-PCR) validation.

Briefly, cDNA templates for real-time PCR were prepared by diluting 1 100 with 10 mmol/l Tris (pH 7.5).

DNA isolates from immunoprecipitates were used as templates for real-time quantitative PCR amplification using the primer pairs listed in Additional File 1, Table S2.