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Thus PSSC leads to target clusters and structural templates for library design.
Systematic analyses of protein-exposed binding epitopes help to develop privileged templates for library design that address protein family members specifically and selectively.
Transposon sequences from these plasmids were used as templates for library alignments.
DNA synthesis from these primers ultimately generates fragments containing primer sequences at both ends, which provide the templates for library preparation with later PCR steps using the adaptor sequences as priming sites.
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No insertions were observed when using plasmid DNA as template for library preparation, although deletions occurred at a low level (0.02 0.03%).
The AAV2/8 recombinant virus was used as a template for library screening of viral capsid mutants that would efficiently transduce bipolar cells.
Modifications to the Adli & Bernstein [ 45] ChIP DNA amplification protocol (see Methods) allowed for successful amplification of 1 ng or less of template (Additional file 2: Figure S7), producing up to several hundred nanograms of template for library preparation.
Following analysis of RNA quality and integrity in a Bioanalyzer (Agilent, Palo Alto, CA) (only samples with a RIN ≥ 8.0 were used), equimolar quantities of purified total RNA samples from each of two biological replicates were pooled into one template for library synthesis.
Approximately 1.4μg male and 2.7 μg female total RNA were used as the templates for cDNA library construction using the Accuscript HF Reverse Transcriptase Kit (Agilent Technologies, Cedar Creek, Texas) and SMART primers (Invitrogen, Carlsbad, CA).
A) Gel image showing size distributions of template DNA for library preparation pre and post shearing.
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