Sentence examples for templates for initial from inspiring English sources
This PCR method may be useful for obtaining templates for initial sequencing of novel orthoreoviruses from diverse vertebrate hosts.
All PCR constructions used 26695 genomic DNA as template for initial amplifications.
The original repressilator plasmid, pZS1 (Elowitz and Leibler, 2000) was used as a template for initial characterization and for construction of the OR2* mutant.
The template for the initial amplification reaction was a 5'-STRETCH plus cDNA library cloned in lambda gt10 vector (Clontech BD Biosciences, Mississauga, ONT).
For the crystallographic analyses of TEAS W273E and farnesylated TEAS W273E, the structure of isomorphously crystallized, wild-type TEAS (with residue 273 truncated to CB) provided a template for the initial atomic model, which was further refined (phenix.refine) in Phenix.
Finally, the derived sockets are employed as eye estimates in which DAISMI is employed to detect the stable approximate pupil-center position by averaging the positions of eyeball border vertices which is necessary for initial template orientation of DTBGE and the final movement displacement vector.
R-GECO1 and R-GECO1.2 in pTorPE [ 5] were used as the initial templates for engineering low-affinity red fluorescent genetically encoded Ca2+ indicators for optical imaging (LAR-GECOs).
DAISMI finds the stable approximate pupil-center location by calculating the mass-center of eyeball border vertices to be employed for initial deformable template alignment.
Known ligands with low docking scores were used as initial 3D templates for the shape comparisons with the chemical library.
An initial search for suitable threading templates for three plant RhoGAP sequences (Selmo2a/b, Phypa7 and Ostta1) performed using the ExPDB function of SwissModel [ 105, 106] yielded no reliable templates.
In all cases, template quantification was performed prior to PCR to ensure that >10 initial HA molecules were used as templates for subsequent amplification.
