Sentence examples for templates for in from inspiring English sources

First, the DNA templates for in vitro transcription were produced by PCR amplification of pJFH1 with the corresponding T7-tagged forward primers and the reverse primers listed in Table 1.

Plasmids were used as templates for in vitro RNA transcription with T7 RNA polymerase (Promega).

Templates for in vitro transcription of LhrA and LhrA-Mut3* were prepared as described previously [23].

DNA templates for in vitro transcription reactions were generated by PCR, with one primer containing the T7 promoter sequence (Table S2).

Templates for in vitro RNA transcription were generated by PCR using primers containing the T7 transcription initiation sequence in the 5' oligonucleotides.

The MMLV-RT enzyme was inactivated at 70°C for 15 min. cDNA products were then used as templates for in vitro transcription to generate fluorescent cRNA.

In the present study we report on strain-independent RT-PCR amplification of four HIV antigens to generate templates for in vitro-transcribed RNA.

The PCR products were used as templates for in vitro transcription with the MEGAscript Kit (Ambion) according to the manufacturer's instructions.

These constructs were sequenced and the plasmids digested with BstEII, ethanol-precipitated and dissolved in distilled water, and used as templates for in vitro transcription.

The dicistronic constructs in pC631 plasmid were linearized using Nru I and used as templates for in vitro synthesis of transcripts using MegaScript T7 transcription kit (ambion).

Purified PCR products containing T7 promoters at each end were used as templates for in vitro transcription using the Megascript T7 kit (Ambion) according to the manufacturer's directions.