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There are no existing templates for full participation by a European country that don't require full freedom of movement.
(Other bilateral association agreements exist for countries like Israel and Bosnia and Herzegovina, but these are special cases for non-European or (pre) accession countries). There are no existing templates for full participation by a European country that don't require full freedom of movement.
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mRNA was converted to cDNA using TaqMan® Reverse Transcription Kit (Applied Biosystem, Foster City, CA, USA) which was used as template for full length PCR of NAMPT by RED Extract-N-Amp™PCR RED Extract-N-Amp™PCR
A cDNA pool equally representing each sampled ripening stage was then used as a template for full-ORF VvDXS cDNA amplification.
The SMART II oligonucleotide (Clontech), which has extra G nucleotides at its 3' end, was used to create an extended template useful for full-length cDNA enrichment.
The template for the full-length STX in a closed state consisted of two specific X-ray structures of STX: One from its complex with the neuronal SNARE proteins (PDBid: 3HD7) and another from its complex with the Munc18a protein (PDBid: 3C98).
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An explanation for how picornaviruses protect RNA replication templates and full-length genomes for encapsidation from VPg removal from virion RNA is via coupling RNA replication and encapsidation, as these viral processes have been demonstrated to be coupled in vitro [32], [50], [52], [53], [54]; it is feasible that the capsid proteins shield the nascent viral genomic RNA from unlinkase activity.
cDNA synthesized with UNINEF7' as primer was used as template for the nearly full-length PCR amplification with limiting-dilution methods [11].
In the infected cell, the 49S genomic RNA serves both as mRNA for the translation of the nonstructural proteins and as a template for synthesis of full-length minus strand RNA copies [1].
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CEO of Professional Science Editing for Scientists @ prosciediting.com