Sentence examples for templates for direct from inspiring English sources

The amplified products were used as templates for direct sequencing (Dragon Genomics, Mie, Japan).

The collected positive clones were treated with a zymolyase solution [2.5 mg/mL Zymolyase-100T (Seikagaku America Inc., East Falmouth, MA, USA), 1.2 M sorbitol, 0.1 M Na phosphate, pH 7.4] for 30 min at 30°C, and then used as templates for direct amplification of the prey clone inserts using the following primers: T0017 (5′-TACCACTACAATGGATGATG-3′) and T0018 (5′- GGGGTTTTTCAGTATCTACG-3′).

The dimensions of the structures are 0.43 ± 0.15 μm in diameter and they have then subsequently been used as templates for directing the synthesis of silica silver and silica polyanthranilic composite hollow spheres.

In summary, we have synthesized flexible chiral cyclodextrin-based templates, and found that they are as effective as rigid templates for directing the synthesis of porphyrin nanorings.

The discovery that strict preorganization is not necessary will make it easier to design templates for directing the formation of new macrocycles.

Since oligonucleotides have become popular as templates for directed changes following induction of a DSB by Cas9 and other gene editing tools [ 2, 31] we sought to determine if NU7441 or KU-0060648 could also stimulate HDR mediated by these much shorter, single-stranded DNA templates.

Although the first Plowshares had been a disappointment, a new template for direct action had been created — one that inspired more than a hundred similar break-ins.

These samples were used as a template for direct DNA sequencing.

Double stranded PCR products were cleaned with Exo-Sap at 60°C for 60 minutes and used as template for direct cycle-sequencing with dye labeled terminators (Applied Biosystems).

PCR products were purified by using ExoSAP-IT treatment (USB Corporation, Cleveland, OH, USA), and were used as template for direct sequencing.

PCR products were purified using a QIAquick PCR Purification Kit (QIAGEN, Germany) as per manufacturers instructions, the DNA quantified with a NanoDrop spectrophotometer ND-10000 NanoDrop Technologies, USA), and 10 20 ng of product used as a template for direct sequencing reactions.