Sentence examples for templates for complementary from inspiring English sources
The obtained tombusvirus replicase is template dependent and it can use added TBSV-derived RNA templates for complementary RNA synthesis, including or RNA synthesis depending on the polarity of the added RNA template [34], [35], [67].
When two-step qPCR is to be run, instead of diluting RNA isolates 1 10 post-DNase treatment, they are each diluted to 59.4 ng/µl and used as templates for complementary deoxyribonucleic acid (cDNA) synthesis (for use as samples or Stock I cDNAs in two-step qPCR), we use SuperScript™ III RT enzyme (Invitrogen) for reverse transcription.
In the genetic system a parental DNA strand acts as a template for complementary activated nucleotides.
This solubilized tombusvirus replicase can use exogenous (added) template for complementary RNA synthesis only [34], [35].
Total RNA from human and mouse keratinocytes were extracted with TRIzol reagent (Invitrogen) and were employed as a template for complementary DNA (cDNA) synthesis performed with SuperScript Vilo (Invitrogen) according to manufacturer's instructions.
Thus, miRNAs are used by RISCs as templates for recognizing complementary mRNA to regulate gene expression.
When the vector is introduced into a bacterial cell and replicates, the mutated strand will act as a template for a complementary strand that will also be mutant, and thus a fully mutant molecule is obtained.
RISC uses miRNA as a template for recognizing complementary transcripts.
Briefly, for construction of lentiviral shRNA-expressing vectors, the hairpin shRNA templates of complementary oligonucleotide containing overhangs were digested.
Equal amounts of mRNA template were used for complementary DNA synthesis using the High Capacity cDNA Kit (Applied Biosystems).
Viral RNA replication reactions were performed in the cell-free viral RNA synthesis system using mnRNP and either v53 (lanes 1 5) or c53 (lanes 6 10) as viral model templates for vRNA and complementary RNA (cRNA), respectively.
