Again, the second verse loses its way slightly, Adams refusing to deviate from his set template for the song even when the original calls for it.
Thus, rationally functionalized materials, be these imprinted by the analyte of interest itself or possess affinity towards it, are able to distinguish the template from interfering species even when having related geometrical and chemical structures.
The reaction between azides and unactivated terminal alkynes is very slow in the absence of CuI catalysis, even when templated by DNA.
Even when all templates sharing >10%% sequence identity with the target were removed (to simulate the lack of similar templates that frequently occur in real world simulations), reasonable predictions for the experimental protein structures were still possible with the average ligand similarity of 0.40 and MCC of 0.28.
Application of this method permitted specific genotype identification for all cases with no cross-reactivity, even when both templates were added to the same tube.
To address this issue, bialleic markers like single nucleotide polymorphisms (SNPs) and insertion-deletions (indels), which can be scored even when the template DNA is heavily degraded (<100 bp), have been suggested as alternative markers for HID testing.
In our case, failure to amplify DNA from particular plant species was a persistent problem even when the template DNA was extracted from fresh tissue and spectrophotometric analysis indicated high DNA yield and quality.
The presented results show that predicted structures can be successfully used for virtual ligand screening, and by exploiting the ideas of LHM, diverse novel small molecule binders can be identified even when the closest template is distantly related to the protein target of interest.
Subsequently, we have shown that this approach can be used to identify functional sites in remote (e.g.10 20% sequence identity) homology models, even when the structural template used to build the model is itself un-annotated (Standley et al., 2008a).
Our structure of the thrombin·boophilin complex and its comparison with the related thrombin·ornithodorin heterodimer reveals that caution must be exercised even when a highly homologous template for the expected complex does exist.
