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Remarkably, ChIP analysis showed that chromatin occupancy by Pol I and Pol III (as represented by the core components RPB6 and RPB8) did not change perceptibly in cells with inactivated RNase P (Figure 6C; also refs. 10 and 11), an indication that these two polymerases can be recruited to their corresponding templates, even though there is no active transcription.
Interestingly, the hPolκ error rates at positions downstream from the lesion site were nearly unchanged between the damaged or control templates, even though the nucleotide incorporation rate of hPolκ was reduced at these positions within the primer extension assays.
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As a first step toward understanding the evolutionary potential of GNA, we have recently shown that Bst DNA polymerase can catalyze faithful (but limited) DNA synthesis on a GNA template even though the DNA product cannot form a stable duplex with the GNA template [6].
The models were built using fitted EM structure as a template, even though high resolution crystal structures were also used simultaneously the interfaces between the different components of the cTF assembly would have unfavourable short contacts.
"Even though, in many ways, it is".
The possibility of forensic use of the recorded fingerprint templates exists, even though no actual fingerprint image is stored, it might be possible to derive a fingerprint template from a latent fingerprint image and match such a template to the stored templates.
In contrast, Y766A gave no observable fingers closing with the complementary dNTP at either a template A or a template T even though this mutant performs step 2.1 with approximately WT kinetics.
Strangely enough, no binding studies have been reported for primer-template DNA (p/t-DNA) even though PrimPol performs DNA synthesis on these types of substrates.
Because recognition templates develop from early associations with familiar kin (and/or with self), familiarity-based recognition should precede phenotype-matching recognition even though one template is thought to be used for both mechanisms.
"#WithoutTenure I can be fired for.... .. was the Tweet template of the day, and even though I rode that bus for a bit, it occurs to me this morning that it misses the point.
Texas Governor Rick Perry, meanwhile, is proving that the right wing governing template simply doesn't work; even though Texas has the lowest government spending in the country, it's still on track to producing a budget deficit projected to be as much as $29 billion.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com