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Templates containing all metadata information from the set-up can in addition be exported as an XML file, which can be shared between researchers and re-imported, thus allowing for comparison between different set-ups and promoting data exchange and dissemination.
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Non-template controls containing all reagents minus the DNA template were included, to monitor the progress of template-independent ligation.
Each real-time PCR run included reactions with no template controls containing all PCR components but without template DNA to ensure that the reagents mix were free of contaminants.
Each PCR run also included non-template controls containing all reagents except cDNA, which generated no amplification.
PCR is simplified in recent years by the availability of reagent kits that allow a forensic DNA laboratory to add a DNA template to a premade PCR mix containing all the necessary components for the amplification reaction.
Blank control tubes containing all reagents except template DNA were included for each run; each sample was analyzed at least twice.
Δ Rn is defined as the difference between Rn+ (Rn of a reaction containing all the components including template) and Rn− (Rn of a no template control).
Water instead of DNA template was used for PCR-negative controls containing all other PCR components.
The sample containing all reagents except the template DNA was treated as the negative control.
Tubes containing all of the ingredients except templates were included in all runs and served as negative controls.
Water containing all the PCR components except template DNA was used as the PCR-negative control.
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