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The templates contained a heading soliciting further information in the referral about each item listed in the subsequent list of items.
Those based on oat R gene templates contained a high frequency of inosines (>15%) and predominantly amplified retrotransposon-like sequences (data not shown).
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Cross-linking experiments were also carried out with templates containing a single BrdU (CTB and CBT templates shown in Figure 1A).
We prepared 5 separate mRNA templates containing a C-terminal FLAG or His6 tag for purification; this set of templates is similar to but more complete than that previously described [1].
Bridging can occur if, and only if, the templates contain a region of sequence similarity.
Primer extension studies were performed in order to investigate the ability of hPol κ to synthesize DNA from templates containing a single M1dG lesion.
DNA templates containing a single consensus promoter for T7 RNAP (+1), or a single null mutant promoter (cross), were immobilized on a surface, with the promoter directing transcription towards the free end (+295).
The fraction of templates containing a bound Gal4DBD remained the same before and after the remodeling reaction, indicating that SWI/SNF with ATP alone is not able to displace a bound Gal4DBD.
After bisulfite conversion, the 156-bp PCR-2 products of methylated Alu templates contain a 5'-G CgnG Cg-3'sequence (including CpG sites H and J), that can be digested by Cac8I.
The fraction of templates containing a bound Gal4DBD was comparable to that of a template without a nucleosome and without SWI/SNF and ATP added, indicating that in the absence of ATP, SWI/SNF is unable to evict a bound Gal4DBD even in the presence of a nucleosome adjacent to a bound Gal4DBD.
Linearized templates containing a 716-bp fragment of human HIP cDNA (bases 521 1236 of human HIP coding sequence) or a 777-bp fragment of human vWF cDNA (bases 7301 8077 of human vWF coding sequence) were radiolabelled with P-dUTP by in vitro transcription using the MAXIscript kit (Ambion).
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