As predicted by macrorestriction and DNA microarray experiments, PCR amplification generated two fragments (fragments [1-2] and [3-4] ) when Pl genomic DNA was used as the template, and one fragment (fragment [1-4]) when TT01α genomic DNA was used as the template, confirming the occurrence of a deletion event in the TT01α genome.
Indeed, our in vitro analyses of the binding requirements using immobilized templates confirmed that methylation of cytosine residues within the IGF2BP1 sequence does not affect CTCF binding.
Successful amplification of house keeping genes using cDNA as the template further confirmed the quality of cDNA.
Examination of cerebral blood flow changes associated with pitch naming minus baseline in the AP automatic template group confirmed the same significant activation peak in the posterior extent of the left superior temporal gyrus (−61, −31, 5) with no associated changes in right frontal pitch working memory structures.
Empty vector was the single most abundant clone in the metagenomic library prior to selection, and the lack of its amplification using post-selection DNA as a template confirmed that the secretome selection step eliminated most of the "background" non-secretome-encoding recombinant phagemids, including the empty vector.
As such, the decrease of PEI decomposition temperature could be ascribed to its well dispersion by the template agents, which confirmed the BET results.
Hence, the DNA phosphatase treatment restored the signal of DNA depurination which was nearly absent from previous tSMS analyses of ancient DNA templates, confirming that modification of 3'-ends, in particular phosphorylation, precluded efficient poly-A tailing of ancient DNA templates with terminal transferase.
Thermally evoked action potentials were additionally used as templates to confirm the selected spikes.
After random correction, three restriction fragments were removed that returned aberrant values (HoxD: chr2:74'597'000-74'597'732; cHoxB74'608'796-74'609'312, HoxB: chr11 95'999'958-96'000'916) due to sequence abnormalities in the BAC template (confirmed by Sanger sequencing; not shown).
Using the DNA present in these immunoprecipitates as templates for qRT-PCR confirmed the association of β-catenin with the IFN-β promoter region.
