Your English writing platform
Discover LudwigSuggestions(2)
Exact(2)
High recombination frequencies are assumed to be related to the presence of partial cDNA that results from incomplete cDNA synthesis as well as partial RNA templates caused by degradation.
This indicates that despite the same amount of DNA used for each sample that the reduction of available templates caused by fragmentation can lead to a higher probability for sequence artefacts to be observed after PCR amplification.
Similar(58)
Other reasons could be possible errors introduced by structural differences between the template and target structures not picked up by the homology modeling procedure, and structural artifacts in the template caused by protein engineering or crystal packing.
Another possible cause of variant repeat synthesis could be the change in telomerase template usage caused by mutation in the catalytic telomerase subunit TERT (Sykorova, Leitch, et al. 2006).
It is also possible that these pauses on WT template are caused by yet unknown interaction of RNAP with nucleic acids scaffold (such as interactions of RNA exit channel with the transcript), which is altered by introduction of the ST sequence.
It was previously reported that the introduction of a low-"G" linker sequence in the TS oligo or the use of an in silico post-alignment filter could reduce barcode-specific biases resulting from strand invasion artifacts [ 9] -- that is, reads produced from premature template switching caused by the TS oligo "invading" the growing cDNA strand due to sequence complementarity.
The huge number of reads from the HTS profiles of sRNAs contain miRNAs that are different from their DNA template, which may caused by either mutations in DNAs or editing on RNAs.
This approach both simplifies the synthetic process and protects the nanotubes from destruction caused by template removal.
This is likely caused by template-switching resulting from incomplete extension of the DNA strand as illustrated in Figure 1c.
It should be noted that generation of the chimeric products is likely caused by template-switching during PCR rather than by new point mutations.
This drop in the template melting temperature is caused by the excessive presence of T and A bases in the RRBS libraries.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com