Exact(1)
A number of studies also associate Smc5/6 with repair of stalled/collapsed replication forks since replication through damaged templates caused an accumulation of X-shaped DNA structures in smc5/6 mutants [ 5, 6, 7].
Similar(59)
The addition of NU7441 or KU-0060648 to cells following introduction of the Cas9/ sgGFP editing system and the ΔGFP repair template caused a decrease of approximately 40%% in NHEJ events which was accompanied by an approximately two-fold stimulation in HDR (Fig. 1f, g).
The rough structure of lotus leaves (hills and valleys template) causes a reduced contact area with water.
A reinvasion upstream from the dissociation site leads to the formation of short tandem repeats within the neosynthesized copy, whereas a downstream reinvasion site that corresponds to a jump from the template causes a gap delimited by microhomology sequences within the neosynthesized copy (Fiston-Lavier et al. 2007).
This indicates that despite the same amount of DNA used for each sample that the reduction of available templates caused by fragmentation can lead to a higher probability for sequence artefacts to be observed after PCR amplification.
With the same concentrations of metallic ions in the precursor solutions, the selection of Ag templates caused quite different nanostructures, i.e., AuAg alloy hollow structure, more precisely, nanorings when Ag NPs were adopted as templates, and Ag@AuAg core-shell NWs in the case of Ag NW templates.
High recombination frequencies are assumed to be related to the presence of partial cDNA that results from incomplete cDNA synthesis as well as partial RNA templates caused by degradation.
Streptavidin pre-bound to a biotin residue in the template caused RT to stall two to three nucleotides upstream from the biotin residue.
An artificial barrier created by binding streptavidin (SA) to a biotin residue in the template caused RT primer extension to stall two nucleotides upstream from the biotin residue indicating that RT protrudes at least two nucleotides beyond the end of the primer.
As the template caused the formation of primary macropores the activation created a secondary system of micropores which resulted in a significant increase of the specific surface area up to about 1400 m2 g−1.
HR efficiency is influenced by template accessibility, causing an up regulation of HR during S and G2 phases of the cell cycle when sister chromatids are available [ 52].
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