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9.2) Spiking events - Were place cell sequences (templates) calculated for all simultaneously recorded CA1 cells or only for that subset that showed a linear progression?
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The place cell sequences (templates) were calculated for each direction of the animal's movement and for each run session in all experimental conditions by ordering the spatial location of the place field peaks that were above 1 Hz.
The quantity of the size selected library was determined by a real-time PCR approach using the Ion Library Quantitation Kit (LT) following the manufacturer's recommendations, with the template dilution factor calculated for a final concentration of ∼26 pM per target.
Since the qualitative data in Figure 2 suggested that there were large differences in the efficiencies of the extension steps, especially for the adducted templates, bypass efficiencies were calculated for each of the three substrates.
The cross-correlation between the template and tomogram is calculated for each position and every possible orientation.
IHSD Template values have been calculated for remote area, non-remote area, and national average decision contexts, to account for regional differences in costs and service provision.
Scans were then transformed into standard space by registration to the Montreal Neurological Institute template and hemispheric volumes were calculated for each individual.
(6) Using the linear prediction model is calculated for user's template in database.
To assure that solely the selected component matched the template, a two-sample t-test was calculated for the best-fit and the second best-fit component.
A homology model was calculated for HM1.24-ETA′ by modeling the scFv (template: 1H8N) and ETA′ (template: 1IKP) separately using the YASARA Structure software (YASARA Biosciences, Graz, Austria).
These similarity scores were calculated for each region under consideration because the template for a candidate fragment would not necessarily be the same as the template for the full-length candidate sequence.
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