Sentence examples for templates by replacing from inspiring English sources

Additionally, we have expanded the range of drug selection options that can be used for PCR-mediated homologous replacement using pRS plasmid templates by replacing the G418-resistance kanMX4 cassette of pRS400 with MX4 cassettes encoding resistance to phleomycin, hygromycin B, nourseothricin, and bialaphos.

The absence of DNA template was verified by replacing the RT/Taq mix by the Taq DNA polymerase only.

No template controls were included by replacing cDNA with DNase free water.

This template nesting feature is exemplified by replacing the "image" component type of the Button-Text-Image template of Sect.

New sgRNA template primers can be made by replacing the N20 in the following sequence with 20 bp of target specific sequence: 5′-cctcctattgcgagatgtcttg(N20 gtttaagagctatgctgg-3′.

Negative controls were prepared by replacing the mRNA template with PCR-grade H2O.

A negative control was incorporated by replacing the cDNA template with Molecular Biology grade water (VWR International).

A negative control was incorporated by replacing the cDNA template with Molecular Biology grade water (VWR International, Lutterworth, UK).

PCR reactions were performed in duplicate, and negative controls, prepared either by replacing the cDNA template with water or without addition of the reverse transcriptase, were amplified in each run.

The template for the PCR, pKT-yEmCherry-CaURA3, was constructed by replacing mCherry sequences in the plasmid pKT-mCherry-CaURA3 with yEmCherry sequences amplified from pNEB31 (Silva et al. 2012) with primers yEmRFP_F and yEmRFP_R (Table S2).

RegaDB can export patient details into a report document by replacing variables in a user-designed RTF template.