Sentence examples for templates by performing from inspiring English sources

We then enriched for cDNA templates by performing multiplex incorporating PCR reactions (≤18 cycles), and isolating 250 550 base pair fragments by gel purification.

The linear range of PCR templates was determined by performing a 10-fold serial dilution standard curve, which usually proved a 1 10 dilution was sufficient.

The BLAST algorithm is used for template structure identification by performing searches against the Protein Data Bank [ 41].

The 20- μm thick crossed nanoporous templates have been prepared by performing a sequential multi-step exposure of energetic heavy ions, at various angles with respect to the normal of the PC film surface.

cDNA templates were then amplified by performing polymerase chain reaction (PCR; 18 cycles) that extended the adapter and incorporated a different six base pair index into each sample.

By performing template stripping using a cylindrical roller, it is possible to control the bending angle of the flexible substrate (via the roller diameter) and the speed of template stripping (rolling speed) precisely.

The contents were made up to 100 μl with MilliQ water before incubating the tube at 72° for 10 min. An aliquot of 10 μl of the reaction mixture was then taken as a template to amplify attB by performing PCR using the primer set DSF003/DSF004 with HindIII recognition sites at the 5′ end.

The method developed by Ertas et al. [8] segments breast regions using a cellular neural network and detects lesions by performing 3D template matching on the normalized maximum intensity time ratio maps.

DNA template integrity is then checked by performing RT-qPCR on a housekeeping gene (β-actin or RNAseP [Ribonuclease P]).

To assess whether the mix assemblies are good representatives of the individual data sets, we compared the former with the latter by performing a template transcriptome assembly in NGen.

A negative control without the cDNA template was included, and contamination by genomic DNA was ruled out by performing PCR analysis on template where reverse transcriptase had been omitted in the RT reactions.