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The sepharose beads with the single stranded templates attached were released into a PSQ 96 Plate Low™ (Qiagen, Hilden, Germany) containing a mix of 12 µL annealing buffer (Qiagen, Hilden, Germany) and 500 nmmol/L of the corresponding sequencing primer (see Table 2).
Sepharose beads with the single-stranded templates attached were added to a PSQ 96 Plate Low™ (Qiagen, Hilden, Germany) containing a mix of 11 mL annealing buffer (Qiagen, Hilden, Germany) and 800 nmmol/L of the corresponding sequencing primers (Metabion, Martinsried, Germany) with following sequences: fragETV6-seq: 5′-GATTTG TAGATTT-3′; fragCDKN1B-seq: 5′-GATTAGTTAATTTTT-3′.
The sepharose beads with the single stranded templates attached were released into a PSQ 96 Plate Low™ (Qiagen, Hilden, Germany) containing a mix of 12 μL annealing buffer (Qiagen, Hilden, Germany) and 500 nmmol/L of the corresponding sequencing primer (see Additional File 9).
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This paper describes the design, synthesis, and structural evaluation of a pair of compounds, comprising molecular templates and attached peptide strands, that mimic a small three-stranded mixed β-sheet (6a and b).
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A template report attached to the memo asks agencies to describe, quantify, and monetize anticipated cost-savings from these actions.
Ideally, during this process a single template is attached to each bead leading to uniform clusters on each bead.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com