The higher incidence of PCR recombination in the amplicon templates is consistent with previous findings of increased rates of PCR recombination with increased concentration of template DNA [42], [43].
Since there were a number of templates with similar E-values and since the homology was remote, an evaluation of the models themselves was carried out using the statistical potential DFIRE [43] and the top four were structurally aligned to each other to determine whether the models based on different templates were consistent with each other.
Allowing newly synthesized positive-sense genomes to remain in the complex and act immediately as replication templates is consistent with previous reports indicating a coupling between translation and replication as we still require initial, infecting genomes to be translated before transitioning to replication (Novak and Kirkegaard, 1994).
The results, shown in Figure 2, indicate that the reaction using FPE RNA as a template was consistent with that seen for the high quality commercial RNA.
When a search template is consistent across trials, thus not requiring the generation and coordination of a to-be-searched-for template with the deployment of spatial attention throughout the display, loading WM has no effect on search performance (Woodman et al., 2001a, 2001b).
We developed templates that are consistent in scope but flexible enough to represent the differences among the projects and their alternatives.
As shown in Table 1, the observed masses for single glyceronucleotide or deoxyribonucleotide incorporation on Template A are consistent with the calculated values.
The fragment specific estimates of template numbers are consistent with the estimates based on limiting dilution (see Figure 9, rank correlation around p = 0.5 for most of the fragments), but indicated that variant recovery in fragment F5 was sometimes poor, consistent with the difficulties we encountered when amplifying fragment F5.
Our observations of increased mutation rates when using highly diluted templates in PCR are consistent with a previous report that low copy number template can decrease the fidelity of both Taq and Pfu DNA polymerases [ 27].
The goal of this study was to determine if current Canadian IC templates and accompanying IRB instructions are consistent with the ethical challenges posed by the rise of NGS technologies in the aforementioned contexts.
