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Each of these artificial β-sheets is composed of two different molecular templates and two dipeptide strands.
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Of note, these gradient and layered structures can be prepared without external triggers or pre-existing templates, and two independent chemistries for cross-linking and patterning purposes are not required.
For each experiment two replicates of each of the seven plasmid templates and two replicates of three cDNA samples from both strains, all synthesised from independent RNA extractions, were used.
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Instead, two templates and three specifically designed primers were applied.
Fixed bases (maximum of six bases for short templates and twelve bases for long templates, Table S1 red) in the barcode region were used for filtering: barcodes which had at least one mismatched fixed base were removed.
Both positively charged hydrocarbon (HC) surfactant cetyltrimethylammonium bromide (CTAB) and fluorocarbon (FC) surfactant FC-4 were used as the templates, and four silica sources tetramethyl orthosilicate (TMOS), tetraethyl orthosilicate (TEOS), 1,2-bis (trimethoxysilyl) ethane (BTME) and 1,2-bis (triethoxysilyl) ethane (BTEE) were used as the precursors.
DT-framework, TT-framework, FT-framework mean our proposed framework using double templates, three templates, and four templates, respectively.
The primary structure multiple alignment of AoXyn11A with three homology modeling templates and seven thermophilic GHF11 xylanases.
Five different sequences were uncovered from the two-template PCR reaction; one copy of each of the two templates and three recombinant sequences.
Nitrogen-doped ordered mesoporous carbons (N-OMCs) were synthesized by nanocasting using SBA-15 silica as template and two polyaramide precursors: m-aminobenzoic acid (MABA) and p-aminobenzoic acid (PABA).
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