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Deposition of silica layer on latex or colloid templates and subsequent removal of the hard templates by calcination or corrosion has been known to be the conventional approach for preparation of HMSNs [5 7].
The microshells, constructed by alternate adsorption of alginate sodium (ALG) and chitosan (CHI) onto the surface of colloidal templates and subsequent removal of cores, allow the polystyrene (PS) or SiO2 nanoparticles (NPs) adsorbed into the internal shells through a simple mix process, as confirmed by confocal laser scanning microscopy (CLSM), and scanning electron microscopy (SEM) analysis.
By varying the amount of divinylbenzene (DVB) in the HIPE templates and subsequent polymers, the BET surface area and micropore volume of the hypercrosslinked analogues can be varied systematically, allowing for the production of carbon foams, or 'carboHIPEs', with varied surface areas, micropore volumes and pore-size distributions.
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Here, we report on a facial route to synthesis ultrafine hollow silica nanoparticles (the diameter of ca. 24 nm) with high surface area by using cetyltrimethylammmonium bromide (CTAB) and sodium bis 2-ethylhexyl) sulfosuccinate (AOT) as co-templates and subis 2-ethylhexylng treatment.
Glassy carbon nanowires were fabricated, for example, by the pyrolysis of poly furfuryl alcohol nanowires polymerized in the pores of a nanoporous alumina template and subsequent template removal [13].
Ni and Co doped Fe2O3 hollow microspheres of yolk-shell type were designed and synthesized by using resin (RF-COOH) microspheres as hard template and subsequent calcination.
It is worth noticing that the deposition time must be controlled to suppress the outer grow of depositing material from the AAO template and subsequent cap formation [30, 31].
The transfer of (110) SiNMs (even unstrained) to a (001 -oriented Si template and subsequent overgrowth can be used to fabricate mixed-crystal-orientation templates[5001 -orientedet al., unpubliSied).
First strand cDNA was generated using the Superscript II system (Invitrogen) from 1 µg of RNA template and subsequent PCR performed using 1 µg of cDNA and zeolin primers ZeF and ZeR.
PCR reaction using bisulfite-treated genomic DNA as a template and subsequent cloning and sequencing was carried out as described in the previous study [11], except that the pSTBlue-1 vector (Novagen) was used for cloning.
Sequencing of each template and subsequent analysis using Mutation Surveyor software (SoftGenetics, State College, PA) was outsourced to MWG Biotech (MWG Biotech AG, Ebersberg, Germany).
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