Sentence examples for templates and specific from inspiring English sources

This critical temperature differs from the templates and specific primers.

The inactivation cassettes were then PCR amplified by using the pGEM3Zf constructs as PCR templates and specific oligonucleotides, containing the sequences of attB1 and attB2 sites, as PCR primers.

The ftsZ gene was amplified by PCR using the chromosomal DNA of S. suis 05ZYH33 as template and specific primers described in Table 2.

PCR products were obtained utilizing E. coli K4 chromosomal DNA as a template, and specific oligonucleotide primers as indicated in the "Materials and methods" section.

In each shrimp sample (n ≥ 6), actively transcribed genes from IHHNV and IMNV were quantified by absolute qPCR using cDNA as the template and specific primers as described in Table 1.

The previously described mutants Rab5S34N and Rab5Q79L [19] were generated by site-directed PCR mutagenesis using Rab5bwt cDNA as template and specific primers harbouring the mutation concerned.

Taqman miRNA Reverse Transcription kits (Applied Biosystem) were used for reverse transcription reactions with 10 ng total RNA as template and specific primers from the Taqman miRNA Assay Kits.

The NSs gene of GBNV-To (K) was amplified by RT-PCR using partially purified viral RNA as template and specific sense and anti-sense primers, designed on the basis of the genomic sequence of S RNA of GBNV (accession number U27809, table 1).

Each inner PCR reaction was performed with 32 replicates (40 μl each) using 5 μl of pooled first round template, and specific primers that incorporated a 4 base identification tag as well as a 19 base 454 adaptor sequence (Table S9).

The PCR pRS300 vector was used as template and specific oligonucleotides were designed (listed in Additional file 4).

Probes were generated by PCR, using the DNA from the same passion fruit accession as template and specific primers.