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LAPs were designed from perennial ryegrass EST templates, and sequence tagged site (STS) primers derived from Italian ryegrass (L. multiflorum Lam).
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Based on independent sets of structural templates and sequences of 127 complete prokaryotic proteomes with optimal growth temperatures from 8°C to 100°C, we performed proteomic homology modeling of complexes and analyzed peculiarities in their traits related to thermal adaptation.
We carried out PCRs using these primers on B. malayi and B. pahangi genomic DNA templates and sequenced the PCR products.
The β-actin gene was used for normalisation of cDNA templates, and sequences of the primes and probe were previously described (Inoue et al, 1997).
Figure 3 shows a structure-based multiple sequence alignment of nitrilase from Aspergillus niger with selected templates and sequences of previously published homology models of nitrilases from R. rhodochrous J1 [ 12, 20] (identity 38%) and Pseudomonas fluorescens [ 1] (26%).
We acknowledge the dedicated efforts of the quality systems, library construction, template, and sequencing teams at the Joint Technology Center of the J. Craig Venter Institute.
Eleven of these were isolated using degenerate primer PCR with cDNA template, and sequences of the remaining five were generated as part of a B. betularia transcriptome sequencing project.
A total of 7 DMRs were amplified from the bisulfite-treated genomic DNA template and sequenced.
In brief, pyrosequencing method included preparation of the template and sequencing reaction.
However, nanopore sequencing is at the moment noncommercialized (offered only through the MinION™ Access Program) and is still being optimized on case-by-case basis using specific template and sequencing needs.
The error of a template-based model comes from template selection and sequence-template alignment, in addition to the structure difference between the sequence and template.
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