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The as-synthesized material was calcined from room temperature to 550°C at a heating rate of 2 6°C/min for 5 h to remove the templates and obtained fine mesoporous SBA-15.
Here, the refined comparative model comprised regions that could not be aligned to any of the templates and obtained unacceptably low scores in all models.
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In order to build reliable homology models and analyze the structural context of residues under selective pressures, we used the HHpred web-server (http://toolkit.tuebingen.mpg.de/hhpred) [ 76] to identify suitable templates and obtain their sequence alignment with the target protease protein sequences.
In this study, we use the amphiphilic lipopolysaccharides, derived from Pseudomonas aeruginosa as imprinting template and obtained an evenly distributed sub-40 nm polymeric nanoparticles by using inverse emulsion method.
The PN used the care-plan template and obtained current physical measures and reviewed recent pathology results.
This is emphasized by aligning the structures from all the other clan CD families with the caspases (using caspase-7 as a template) and obtaining a measure of structural similarity (PDBeFold [ 66], as described above).
In the detection stage, the templates and parameters obtained in the training stage are used to detect EPSCs.
To confirm TI-NGS assay's variant calls at the discrepant positions with Sanger sequencing, we custom designed template-dependent NGS assays and obtained 100% concordance with the TI-NGS assay.
We built SMURFLite templates for these 207 superfamilies, and obtained from UniProt the protein sequences for T.maritima, comprising 1852 genes.
We built SMURFLite templates for these 207 superfamilies, and obtained from UniProt the protein sequences for each of 1852 genes.
Finally, the high-resolution scanning electron microscopy (HRSEM) JEOL JSM-7800F was used to analyze the morphology of the composite templates and to obtain EDS maps (on MPS-HA sample).
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