As suggested by the reviewer, we have now performed continuous labeling experiments using the G-less cassette DNA template, and observed transcripts of different lengths that provides independent and complementary evidence for transcription reinitiation in our system.
An initial S1 nuclease cleavage assay to determine the effects of 'bubble' size on cleavage sensitivity demonstrated that differential cleavage between the wild-type and mutant templates was observed for some, but not all, annealed oligonucleotides.
For instance, signals from LAMP zones with H. influenzae and Streptococcus pneumoniae (S. pneumoniae) DNA templates were observed to be similar to NC in Figure 4, in contrast to bright fluorescence from N. meningitidis, which confirmed the high specificity of our approach in the detection of N. meningitidis.
Increased amplification of DNA templates was observed following WGA; however, no amplification advantage for ssDNA ligase treatment of templates was evident compared to linear templates.
This is the first time that the morphology of semi-crystalline diblock copolymers infiltrated within AAO templates is observed.
After electrodepositing the Cu nanowires at different temperatures, the cross sections of the nanowire-embedded AAO templates were observed to determine the large-scale length uniformity.
Furthermore, these variants negatively affected error-free replication across damaged templates, as observed by a reduction in the X structures representing damage-bypass SCJs.
Injecting this Cas9 plasmid (pJW1259), the PCR-generated pha-1 and nhr-23 P U6:: sgRNA templates, the pha-1 (ts) sense 80mer repair template, and the nhr-23 :2×FLAG sense 200mer, efficient knock-in at both loci with comparable frequency to the corresponding plasmid-based P U6::sgRNA templates was observed.
As shown in Additional file 1, Figure S5, significant allele-specific amplification bias for gDNA templates was observed for most genes, with occasional genes showing greater than 1.5-fold differences in amplification between alleles.
We found that discrimination between the mispaired C and 5mC templates is also observed with KOD exo-, albeitoto a lower extent than with KlenTaq (47 % extension of the A-primer when paired with 5mC template as compared to 34 % with C template; Figure 1 d).
