Sentence examples for templates and modified from inspiring English sources

The C1392S, the S1394A and the C1392S/S1394A mutants were generated by site-directed mutagenesis with the Quick Change Site-Directed Mutagenesis kit (Stratagene, La Jolla, CA) according to the manufacturer's directions using Flag-HD-PTP full-length and catalytic domain constructs as templates and modified forward and reverse primers described in figure S1.

A variety of the items were based on existing critical appraisal templates and modified accordingly for the development of the ACE tool [ 15, 16].

A fraction of Lpp20, an outer membrane protein of H. pylori known as NQA, was chosen as template and modified with myristic acid to facilitate its localization on the surface of the nanoparticles.

MS had been prepared via surfactant-template method and modified by octyltriethoxysilane (OTS).

Families of mesoporous (2 to 300 nm) metal silicates and nano-oxides and subnanopowders (4 to 300 nm) were synthesized by the template method and modified sol-gel technique, respectively.

The mutated version of this plasmid (p-zbtb7a 3'UTRm) was generated by utilizing the p-zbtb7a 3'UTR as template and modifying the miR-20a seed binding site using the QuikChange II XL Site-Directed Mutagenesis Kit.

The template was iteratively trialed and modified based on feedback from the charge nurses completing the note (Additional file 3).

The structure of these peptides is based on the LPS-binding domain of the Limulus anti-LPS factor as a template and was modified with respect to its capacity to bind and neutralize LPS [ 2- 4].

As in the case for the variable definitions, templates are authored, modified and activated through a web based user interface and all templates are published through a web services interface.

The complex of the Dpo4 polymerase with the 1, N-εGuo lesion (PDB entry: 2BQU) (a refined structure downloaded from Protein Data Bank) was used as a starting model by removing water molecules and modifying the template and primer and inserting the (6 S -HNE-1 S -HNE-1, N-dGuo adduct.

The template is then modified and extended through successive readings until it provides sufficient coverage of the data.