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The primer extension reaction uses PCR products as templates and generates distinct mass signals for competitor and cDNA-derived products.
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Imprinting procedures lead to functional properties of polymeric materials, as the analyte itself is used as a template and generates adapted cavities that are suitable for re-inclusion.
The component traverses a given archetype or template and generates an example RM structure valid to the constraints.
The bacterial RNA polymerase utilizes the 6S RNA as a template and generates short (14- to 20-nt) RNA products that are initiated within the bubble [ 6, 10].
When sequence identity is below 30%, it is difficult to recognize the best template and generate accurate sequence-template alignments, so the resultant models have a wide range of accuracies (Chakravarty et al., 2008; Sanchez et al., 2000).
The oligonucleotide sequences for qPCR are listed in additional file 3. The efficiency for each primer pair (E) was determined by running 10-fold serial dilutions (4 dilution series) of Rm1021 genomic DNA as template and generating a standard curve by plotting the log of the dilution factor against the CT value during amplification of each dilution.
Focusing on the influence of conformational details in the template structures, we based our study on 8 experimentally determined template structures and generated 120 designs from each.
Log Parser can also help create reports and charts that can be used in a report for a company's management team or an outside vendor, using free-form templates and automatically generated pie, line, and bar charts.
To remove the templates and so generate the porous materials, acid extraction on the as-synthesized materials (1.0 g) was performed in MeOH (100 mL) with concentrated HCl (1.0 mL) at 60 °C for 6 h.
BATS also selects the best template for 3D model construction and generates the files for the automated alignment used by the Modeller program [ 42].
For each of the three topologies generated by NAST, we used five models as template starting structures and generated 10 full atomic structures which we then minimized.
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