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In addition, two different-sized cores were used to create templates and demonstrate the size scalability of the process.
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We also tested an IDLV construct for delivery of donor templates and demonstrated its efficacy for gene modification.
This paper specifies the requirements for a Decision Support tool based on the Learning Health System, introduces the theoretical model for provenance templates and demonstrates the resulting architecture.
To facilitate the simultaneous identification of BH3-only targets that are regulated at the RNA level, we have designed and assembled multi-probe ribonuclease protection assay templates and demonstrated their ability to detect multiple BH3-only transcripts in a variety of human cell lines and primary tissues.
A set of EST-SSR primer pairs has been designed using unigene templates and demonstrated to be effective for polymorphism detection within cultivated germplasm and across the genus Lens.
Here, polymeric foams composed of poly ethylene glycol) diacrylate, sodium/calcium polyacrylate and PNIPAM, were synthesized via high internal phase emulsion (HIPE) templating and demonstrated exceptional material properties and biological performance for application as wound dressing materials.
Using a gradient PCR, we identified the Tc for each mutant template and demonstrated that it was correlated to the primer's Tm.
In this paper, we present the implementation of Cytoscape data exporters and template web applications and demonstrate how users can publish their data sets as interactive data visualizations with our new tools.
The annealing rate was determined to be almost the same for both wild-type and mutant complimentary template sequences and demonstrated a decrease in annealing rate as the mismatch 'bubble' size increased.
Here, we report results that provide a comprehensive, quantitative understanding of the impact of multiple primer template mismatches on qPCR accuracy and demonstrate a multi-primer set approach to accurately quantify a model gene pcrA (encoding perchlorate reductase) that has substantial sequence variation.
This method is consistent with previous studies that employed spatial templates to demonstrate replay (Lee and Wilson, 2002; Foster and Wilson, 2006; Diba and Buzsaki, 2007) and preplay (Dragoi and Tonegawa, 2011) during sleep or awake rest.
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