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Strikingly, when replicating various G4 plasmid templates, an additional band of around 750 nt, corresponding to a product stalled close to the G4 site, was visible from 2.5 up to 20 min depending on the replicated G4 sequence (Fig 3C, right panels, and Supplementary Fig S3).
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While embedded in the carbon matrix, these pyrolysis-generated templates undergo an additional phase transformation at the sequential 900 °C pyrolysis, exsolving well-dispersed smaller Fe nanoparticles, typically sizing 5 45 nm, on the MgO surface.
A recent study has shown that continued synthesis and elongation of new DNA primers on ssDNA templates represent an additional key step in checkpoint activation (Van et al, 2010).
All the PFO-DBT nanorods that grown inside the template have an additional diffraction peak at 25.2°.
Use of the primers with heterozygous mutant Nnt C57BL/6J template produces an additional faint product of approximately 900 bp that assists assignment of genotype.
0.1 μl of the PCR-product was then used as template in an additional PCR reaction using the primers fp3: 5'-CAGCAGCGGCCGCTTCCTCTC CTandCG-3' and rp2: 5'-ACAAAGAATTCCGGGGATCTAGCCTGTGG-3' for 30 cycles of PCR, to incorporate the mutations in the full-length gene product.
The rotational reversal was mainly contributed from the underlying nanoporous AAO templates that provided an additional pinning effect.
The final template was somewhat changed from the initial template by including an additional higher-order theme, resulting in three high-order themes; 'strenuous work situation for HCWs', 'economy' and 'coping strategies'.
Template M3 bears an additional heteroaromatic sidechain that can access a subsidiary cleft in one branch of the ligand-binding groove.
In contrast, an incoming dGTP remained in an anti conformation opposite the dT template and made an additional stabilizing hydrogen bond to Gln59 of the finger subdomain of pol ι, thus explaining the preferential misincorporation of dGTP opposite dT.
Detailed biochemical screening showed that majority of the compounds conform to the M3 template, which bears an additional heteroaromatic sidechain that can access a subsidiary cleft in one branch of the ligand-binding groove.
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