Taking these short fragments as templates, a random hexamer primer was used to synthesise first-strand cDNA.
Using these short fragments as templates, a random hexamer-primer was used to synthesize the first-strand cDNA.
Taking these short fragments as templates, a random hexamer-primer was used to synthesize the first-strand cDNA, and then the second-strand was amplified.
It is stored in the form of a template combined with a random string which needs to be found to get the data.
RNA concentration was quantified by Nanodrop spectrophotometry and 500 ng of RNA template included in a random primed cDNA first strand synthesis reaction (Superscript VILO cDNA synthesis kit; Life Technologies).
Each pair of primers was tested on different samples in the same plate to ensure optimal reproducibility and repeated testing was performed on whole template setup or a random selection of samples to ensure inter-assay reproducibility.
IFN-γ, IL-17, IL-4, T-bet, RORγt, GATA-3, Foxp3, TNF-α, IL-6, interferon-inducible protein (IP -10, MCP-1, argIP -101 (Arg1), mannose receptor C-type 1 (Mrc1), IL-10 arginaseeraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA templates were synthesized using a random hexamer primer and RNase H-reverse transcriptase (Invitrogen).
Thus, our proposed CfERV amplification scenario favors a random template master gene model [26] over a strict master gene model [27].
Using this TAS2R16 expression construct as a template, a library of random mutations (Diversify PCR Random Mutagenesis Kit, Clontech) was created using a PCR-based "shotgun mutagenesis" method so that every amino acid position was mutated21, 49.
For all RNA analyses, the total RNA was isolated from treated or non-treated cells using Trizol reagent (Invitrogen), treated with RQ1 RNase-Free DNase (Promega, Madison, WI) to remove residual DNA, and cDNA was synthesized using 2 μg of total RNA as a template, random hexamers, and M-MuLV reverse transcriptase (NEB).
Choose a random, nice looking sample template.
